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Usher Syndrome 1C (Autosomal Recessive, Severe) (USH1C) antibody

Details for Product No. ABIN185190, Supplier: Log in to see
Antigen
  • 2010016F01Rik
  • AIE-75
  • DFNB18
  • DFNB18A
  • harmonin
  • NY-CO-37
  • NY-CO-38
  • PDZ-45
  • PDZ-73
  • PDZ-73/NY-CO-38
  • PDZ73
  • PDZD7C
  • ush1cpst
Reactivity
Human
63
11
8
3
1
1
1
1
1
Host
Goat
40
14
9
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
3
3
3
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
49
42
22
7
4
3
3
1
Supplier
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Immunogen DRKVAREFRHKVD-C
Blocking Peptide Blocking peptide for this product available: ABIN370094
Specificity This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1). Human, Mouse, Rat 652 aa
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name USH1C / Harmonin (USH1C Antibody Abstract)
Background All names and symbols: USH1C, AIE-75, DFNB18, PDZ-45, PDZ-73, HARMONIN, NY-CO-37, NY-CO-38, PDZ-73/NY-CO-38, Usher syndrome 1C (autosomal recessive, severe), harmonin, PDZ-73 protein, ush1cpst, deafness, autosomal recessive 18. Official Symbol: USH1C. RefSeq number(s): NP_005700.2, NP_710142.1
Gene ID 10083, 72088, 308596
Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 75kDa band observed in lysates of cell line HEK293 (calculated MW of 62.2kDa according to NP_005700.2). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney, shows diffuse cytopasmic staining in epithelial cells of renal tubules. Recommended concentration, 10µg/ml. (IHC data have been obtained with EB06287 from previous batch).
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Usher Syndrome 1C (Autosomal Recessive, Severe) (USH1C) antibody (ABIN185190) ABIN185190 (0.1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Pri...
Immunohistochemistry (IHC) image for anti-Usher Syndrome 1C (Autosomal Recessive, Severe) (USH1C) antibody (ABIN185190) ABIN185190 (10 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen ...
Background publications Verpy, Leibovici, Zwaenepoel, Liu, Gal, Salem, Mansour, Blanchard, Kobayashi, Keats, Slim, Petit: "A defect in harmonin, a PDZ domain-containing protein expressed in the inner ear sensory hair cells, underlies Usher syndrome type 1C." in: Nature genetics, Vol. 26, Issue 1, pp. 51-5, 2000 (PubMed).