N-Methylpurine-DNA Glycosylase (MPG) antibody

Antigen: N-Methylpurine-DNA Glycosylase (MPG)

Synonyms: AAG, MDG, APNG, Mid1, anpg, PIG11, PIG16, CRA36.1, Aag, AI326268, 9830006D05, bY187G17.9, si:xx-187g17.9, MPG, MPGR

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185209

Additional Information

Alternative name: MPG

Gene ID: 4350

Immunogen: C-SVVDRVAEQDTQA

Format: Affinity-purified

Description: All names and symbols: N-methylpurine-DNA glycosylase, AAG, APNG, CRA36.1, MDG, Mid1, N-methylpurine-DNA glycosylase, MPG, PIG11, PIG16, anpg, 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p, CRA36.1 (3-methyl-adenine DNA glycosylase), alkyladenine DNA glycosylase, proliferation-inducing protein 11, proliferation-inducing protein 16. Official Symbol: MPG. RefSeq number(s): NP_002425.2, NP_001015052.1, NP_001015054.1. Gene ontology terms: damaged DNA binding activity, DNA glycolase, DNA dealkylation, base-excision repair, nucleoplasm

Specificity: This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28-30kDa band observed in lysates of cell line K562 (calculated MW of 30.8kDa according to NP_001015054.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN185209 staining (2µg/ml) of K562 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Samson, Derfler, Boosalis et al.: "Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9127-31, 1991 (PubMed).