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WD Repeat and FYVE Domain Containing 1 (WDFY1) antibody

Details for Product No. ABIN185214, Supplier: Log in to see
Antigen
  • MGC93914
  • zgc:101764
  • zgc:109909
  • FENS-1
  • WDF1
  • ZFYVE17
  • 1700013B03Rik
  • 1700120F24Rik
  • Jr1
  • mKIAA1435
Reactivity
Human
32
18
16
1
1
1
1
1
1
Host
Goat
19
14
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA
11
11
6
6
3
1
Supplier
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Immunogen Peptide with sequence VVGCSLATGFSPH, from the C Terminus of the protein sequence according to NP_065881.1.
Blocking Peptide Blocking peptide for this product available: ABIN370508
Specificity This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127) Human, Mouse, Rat
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FENS1 / WDFY1 (WDFY1 Antibody Abstract)
Background This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127). Principal Names: ZFYVE17, phosphoinositide-binding protein SR1, WD40 and FYVE domain containing 1, WD repeat and FYVE domain containing 1, KIAA1435, FENS-1, WDF1, WDFY1. Official Symbol: WDFY1
Gene ID 57590
NCBI Accession NP_065881
Application Notes Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave no signal but low background in Human Liver and HepG2 lysates at up to 1µg/ml. IHC: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Recommended concentration, 4-6µg/ml.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Ridley, Ktistakis, Davidson et al.: "FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments." in: Journal of cell science, Vol. 114, Issue Pt 22, pp. 3991-4000, 2001 (PubMed).