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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term)

Details for Product anti-GAPDH Antibody No. ABIN185240, Supplier: Log in to see
Antigen
  • BEST:GH12586
  • cb609
  • CG8893
  • CG12055
  • Dmel\\CG8893
  • Dmel\\CG12055
  • g3pd
  • G3PD
  • G3PDH
  • GA3PDH
  • GADPH
  • gadph
  • GAP
  • GAPC-2
  • gapd
  • GAPD
  • Gapd
  • gapdh
  • GAPDH
  • Gapdh
  • GAPDH-1
  • Gapdh-1
  • gapdh-1
  • Gapdh-2
  • GAPDH1
  • GAPDH2
  • Gapdh13F
  • Gapdh43E
  • GAPDHI
  • GAPDH I
  • GAPDH II
  • GB14798
  • gh12586
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • KNC-NDS6
  • mg:bb02e05
  • T6J4.17
  • T6J4_17
  • wu:fb33a10
  • wu:ft80f05
Epitope
C-Term
110
48
30
25
16
10
10
9
8
7
6
6
6
5
5
5
5
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
657
304
255
137
98
60
58
52
43
41
34
32
32
26
22
17
15
15
14
14
13
12
8
8
8
8
7
6
6
6
6
4
4
4
3
3
2
1
1
1
1
1
1
1
1
1
Host
Goat
361
324
56
31
20
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
46
41
21
13
12
12
10
10
10
9
9
8
6
6
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
710
331
243
161
152
129
44
29
26
22
16
14
10
7
7
6
6
5
5
4
4
2
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Blocking Peptide Blocking peptide for this product available: ABIN369199
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35 kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1 kDa according to NP_002037). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3 µg/mL (data not shown).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term) (ABIN185240) ABIN185240 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term) (ABIN185240) ABIN185240 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...
Product cited in: Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, 2014 (PubMed).

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, 2013 (PubMed).

Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, 2008 (PubMed).

Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, 2008 (PubMed).

Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, 2008 (PubMed).

Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, 2007 (PubMed).

Background publications Burke, Enghild, Martin, Jou, Myers, Roses, Vance, Strittmatter: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).