GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term)

Details for Product anti-GAPDH Antibody No. ABIN185240, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
  • Glyceraldehyde 3 phosphate dehydrogenase 2
  • glyceraldehyde-3-phosphate dehydrogenase
  • glyceraldehyde 3-phosphate dehydrogenase
  • similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • glyceraldehyde-3-phosphate dehydrogenase, type I
  • Glyceraldehyde 3 phosphate dehydrogenase 1
  • glyceraldehyde-3-phosphate dehydrogenase-like
  • olfactory receptor, family 8, subfamily K, member 3-like
  • Gapdh2
  • GAPDH
  • gapdh
  • Gapdh
  • GAPC2
  • gapDH
  • Gapdh1
  • LOC614985
Epitope
C-Term
109
34
32
22
17
10
9
9
8
8
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7
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6
6
6
5
5
5
5
5
5
5
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
744
427
341
161
134
84
78
60
60
58
55
50
49
29
28
26
23
23
18
17
15
15
14
13
10
10
9
7
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6
6
6
6
5
5
5
4
4
3
3
3
3
1
1
1
1
1
1
Host
Goat
391
357
57
31
18
1
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
55
42
22
15
13
13
12
11
11
10
10
8
8
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7
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6
6
5
3
2
2
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2
1
1
1
1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
773
345
299
216
215
164
55
34
28
23
17
16
13
11
7
7
6
6
4
2
2
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Blocking Peptide Blocking peptide for this product available: ABIN369199
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35 kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1 kDa according to NP_002037). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3 µg/mL (data not shown).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody (ABIN185240) ABIN185240 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody (ABIN185240) ABIN185240 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...
Product cited in: Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, 2014 (PubMed).

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, 2013 (PubMed).

Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, 2008 (PubMed).

Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, 2008 (PubMed).

Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, 2008 (PubMed).

Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, 2007 (PubMed).

Background publications Burke, Enghild, Martin, Jou, Myers, Roses, Vance, Strittmatter: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).

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