BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody

Antigen: BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)

Synonyms: BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185253

Additional Information

Alternative name: BIM (AD/ACD/ABCD isoforms)

Gene ID: 10018

Immunogen: C-FNAYYARRLEK

Format: Affinity-purified

Description: All names and symbols: BIM, BCL2L11, BAM, BOD, BCL2-like 11 (apoptosis facilitator), bcl-2 interacting protein Bim, bcl-2-related ovarian death agonist, bcl-2 interacting mediator of cell death. Official Symbol: BCL2L11. RefSeq number(s): NP_619528,NP_996886,NP_619529,. Gene ontology terms: apoptosis, induction of apoptosis, membrane, membrane fraction

Specificity: This antibody is expected to recognise 3 reported isoforms: BimAD, BimACD, and BimABCD Human 169, 79, 109

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 23kDa band observed in K562 lysates (predicted MW of 19, 9, 12kDa according to NP_619528, NP_996886, NP_619529). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185253 staining (0.5µg/ml) of K562lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Marani, Tenev, Hancock et al.: "Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis." in: Molecular and cellular biology, Vol. 22, Issue 11, pp. 3577-89, 2002 (PubMed).