»
Antibodies
»
anti-B-Cell CLL/lymphoma 2 (BCL2) antibody
B-Cell CLL/lymphoma 2 (BCL2) antibody
| Antigen |
|
| Synonyms |
Bcl-2, bcl-2, BCL2 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (145), Mouse (Murine) (57), Rat (Rattus) (55), Cow (Bovine) (37), Dog (Canine) (37), Pig (Porcine) (34), Rabbit (21), Chicken (18), Horse (Equine) (18), Cat (Feline) (2), Sheep (Ovine) (2)
|
|
Application
|
Western Blotting (WB) (96), Immunofluorescence (IF) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Flow Cytometry (FACS) (43), Immunoprecipitation (IP) (36), Immunohistochemistry (IHC) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), ELISA (23), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
|
| Catalog no. |
ABIN185269 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
BCL2
|
|
Gene ID
|
596, 12043 (mouse), 24224 (rat)
|
|
Immunogen
|
AGRTGYDNREIVMKYC
|
|
Format
|
Affinity-purified
|
|
Description
|
All names and symbols: BCL2, Bcl-2, B-cell CLL/lymphoma 2. Official Symbol: BCL2. RefSeq number(s): NP_000624, NP_000648. Gene ontology terms: anti-apoptosis, cell growth and/or maintenance, humoral immune response, integral to membrane, mitochondrial outer membrane, mitochondrion, negative regulation of cell proliferation, protein binding, regulation of apoptosis, regulation of cell cycle
|
|
Specificity
|
This antibody is expected to recognise both reported isoforms (NP_000624, NP_000648) Human, Mouse, Rat 239 aa, 205 aa
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 25 kDa band observed in Human Lymph node lysates (predicted MW of 26kDa according to NP_000624, alpha isoform). Recommended for use at 1-3µg/ml.
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Reed: "Double identity for proteins of the Bcl-2 family." in: Nature, Vol. 387, Issue 6635, pp. 773-6, 1997 (PubMed).
|
Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
|
Hosts
|
Rabbit (124), Mouse (27), Goat (3), Sheep (3)
|
|
Reactivities
|
Human (145), Mouse (Murine) (57), Rat (Rattus) (55), Cow (Bovine) (37), Dog (Canine) (37), Pig (Porcine) (34), Rabbit (21), Chicken (18), Horse (Equine) (18), Cat (Feline) (2), Sheep (Ovine) (2)
|
|
Applications
|
Western Blotting (WB) (96), Immunofluorescence (IF) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Flow Cytometry (FACS) (43), Immunoprecipitation (IP) (36), Immunohistochemistry (IHC) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), ELISA (23), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
|
|
Conjugates
|
PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
|
|
Epitopes
|
pSer70 (5), pThr56 (5), BH3 Domain Specific (1)
|
Alternatives 
