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Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody

Details for Product No. ABIN185308
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Antigen
Synonyms PAD, PAD4, PDI4, PDI5, PADI5, Pad4, Pdi4
Epitope
N-Term
(14), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(61), (7), (7), (1), (1), (1), (1), (1), (1)
Host
Goat
(46), (11), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(51), (25), (16), (13), (10), (8), (7), (5), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
Shipping to United States (Change)
Availability Available
Catalog No. ABIN185308
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence AQGTLIRVTPEQPC, from the N Terminus of the protein sequence according to NP_036519.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAD4 / PADI4
Background Synonyms: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine deiminase, type IV, protein-arginine deiminase, peptidyl arginine deiminase, type V
Gene ID 23569
NCBI Accession NP_036519.2
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 74 kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1 kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody ABIN185308 (0.3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Hosts (46), (11), (4)
Reactivities (61), (7), (7), (1), (1), (1), (1), (1), (1)
Applications (51), (25), (16), (13), (10), (8), (7), (5), (3), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (3), (2), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185308
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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