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Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody

Details for Product No. ABIN185308, Supplier: Login to see
Antigen
  • PAD
  • PAD4
  • PADI5
  • PDI4
  • PDI5
  • Pad4
  • Pdi4
Epitope
N-Term
15
14
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
94
9
7
1
1
1
1
1
1
1
Host
Goat
57
21
15
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
72
28
21
20
13
11
6
5
5
4
2
2
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence AQGTLIRVTPEQPC, from the N Terminus of the protein sequence according to NP_036519.2.
Blocking Peptide Blocking peptide for this product available: ABIN368758
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAD4 / PADI4 (PADI4 Antibody Abstract)
Background Synonyms: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine deiminase, type IV, protein-arginine deiminase, peptidyl arginine deiminase, type V
Gene ID 23569
NCBI Accession NP_036519
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 74 kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1 kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody (ABIN185308) ABIN185308 (0.3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). ...
Background publications Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).