EF-Hand Domain Family, Member D2 (EFHD2) antibody

Antigen: EF-Hand Domain Family, Member D2 (EFHD2)

Synonyms: SWS1, MGC4342, RP3-467K16.3, MGC114423, efhd2, EFHD2, MGC139236, MGC131292, AA408606, D4Wsu27e, 2600015J22Rik, fj19b07, wu:fj19b07, wu:fb80e07, PAD, PAD4, PDI4, PDI5, PADI5

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185311

Additional Information

Alternative name: Swiprosin 1 / EFHD2

Gene ID: 79180, 27984 (mouse)

Immunogen: CAAFKELQSTFK

Format: Affinity-purified

Description: All names and symbols: EFHD2, MGC4342, EF hand domain containing 2. Official Symbol: EFHD2. RefSeq number(s): NP_077305.2. Gene ontology terms: calcium ion binding

Specificity: This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz. Human, Mouse, Rat 240 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 26.7kDa according to NP_077305.2). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded human tonsil stains cells in the proximity of the epithelial criptae, granulocyte neutrophils, macrophages within the germinal centre and in scattered cells (particularly in the interfollicular area and associated with epithelium) that resemble macrophages and/or dendritic cells. (IHC data have been obtained with ABIN185311 from previous batch.)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185311 (0.1µg/ml) staining of human lymph node lysate. Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185311 (0.3µg/ml) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining. Positive cells A) in the interfollicular area and surrounding a vessel, B) in proximity of the epithelial criptae.

Publications

Publications: Vuadens, Rufer, Kress et al.: "Identification of swiprosin 1 in human lymphocytes." in: Proteomics, Vol. 4, Issue 8, pp. 2216-20, 2004 (PubMed).

Blagoev, Ong, Kratchmarova et al.: "Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics." in: Nature biotechnology, Vol. 22, Issue 9, pp. 1139-45, 2004 (PubMed).