HSD17B10 Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) antibody

Antigen: HSD17B10 Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10)

Synonyms: ABAD, CAMR, ERAB, HCD2, MHBD, HADH2, MRPP2, MRX17, MRX31, SCHAD, MRXS10, SDR5C1, 17b-HSD10, DUPXp11.22

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185330

Additional Information

Alternative name: ABAD / HADH2

Gene ID: 3028

Immunogen: CIRLDGAIRMQP

Format: Affinity-purified

Description: All names and symbols: HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase. Official Symbol: HADH2. RefSeq number(s): NP_004484. Gene ontology terms: 3-hydroxyacyl-CoA dehydrogenase activity, 7-alpha-hydroxysteroid dehydrogenase activity, lipid metabolism, metabolism, mitochondrion, oxidoreductase activity, plasma membrane

Specificity: Human, Mouse, Rat, Dog, Bull, Zebrafish, Xenopus, Chimp

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2µg/ml. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185330 staining (0.3µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Yan, Fu, Soto et al.: "An intracellular protein that binds amyloid-beta peptide and mediates neurotoxicity in Alzheimer's disease." in: Nature, Vol. 389, Issue 6652, pp. 689-95, 1997 (PubMed).

He, Schulz, Yang: "A human brain L-3-hydroxyacyl-coenzyme A dehydrogenase is identical to an amyloid beta-peptide-binding protein involved in Alzheimer's disease." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10741-6, 1998 (PubMed).

Chalbot, Morfin: "Human liver S9 fractions: metabolism of dehydroepiandrosterone, epiandrosterone, and related 7-hydroxylated derivatives." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 33, Issue 4, pp. 563-9, 2005 (PubMed).