Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) antibody

Antigen: Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9)

Synonyms: FH3, NARC1, LDLCQ1, NARC-1, HCHOLA3, PCSK9, PC9, Narc1, AI415265, AI747682, MGC47409

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185371

Additional Information

Alternative name: PCSK9

Gene ID: 255738

Immunogen: CRSRHLAQASQELQ

Format: Affinity-purified

Description: All names and symbols: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin\/kexin type 9. Official Symbol: PCSK9. RefSeq number(s): NP_777596.2. Gene ontology terms: cholesterol metabolism, lipid metabolism, negative regulation of enzyme activity, peptidase activity, protein self binding, proteolysis and peptidolysis, subtilase activity

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Detects transfected human pcsk9 in McA cell lysates (see figure) as two bands of approx. 73 and 60 kDa, representing unprocessed and processed protein. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1,14(9):1161-9, PMID: 15772090. Preliminary experiments on untransfected samples gave an approx 35kDa band in Human Liver, Pancreas and Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kDa according to NP_777596.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Metabolism, Amino Acids

Restrictions: For Research Use only

Images

ABIN185371 staining (0.2µg/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.

Publications

Publications: Cohen, Pertsemlidis, Kotowski et al.: "Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9." in: Nature genetics, Vol. 37, Issue 2, pp. 161-5, 2005 (PubMed).

Lalanne, Lambert, Amar et al.: "Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1312-9, 2005 (PubMed).