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Antibodies
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
| Antigen |
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| Synonyms |
ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (139), Rat (Rattus) (65), Mouse (Murine) (63), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Application
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Western Blotting (WB) (132), Immunofluorescence (IF) (34), ELISA (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN185399 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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ERK1 / MAPK3
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Gene ID
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5595
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Immunogen
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GGEPRRTEGVGP-C
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Format
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Affinity-purified
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Description
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All names and symbols: MAPK3, ERK1, mitogen-activated protein kinase 3 , HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44). Official Symbol: MAPK3. RefSeq number(s): NP_002737.1
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Specificity
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No cross-reactivity expected with ERK2 Human 379 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Wang, Weng, Anderson: "OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation." in: Science (New York, N.Y.), Vol. 307, Issue 5714, pp. 1472-6, 2005 (PubMed).
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Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
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Hosts
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Rabbit (114), Mouse (30), Goat (5), Chicken (1), Sheep (1)
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Reactivities
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Human (139), Rat (Rattus) (65), Mouse (Murine) (63), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (132), Immunofluorescence (IF) (34), ELISA (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (1), HRP (1)
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Epitopes
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pTyr204 (8), N-Term (7), pThr202 (7), pThr202/pTyr204 (5), C-Term (4), pThr202,pTyr204 (2), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT-E-pY Motif (1), pT202 (1), pY204 (1)
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Alternatives 
