ADAM Metallopeptidase Domain 33 (ADAM33) (Internal Region) antibody

Details for Product No. ABIN185401
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Antigen
Synonyms ADAM13, ADAM33, Adaml, adam13, C20orf153, DJ964F7.1
Epitope
Internal Region
(3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(15), (3), (2), (2)
Host
Goat
(11), (4), (2)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(15), (7), (3), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN185401
374.27 $
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Immunogen Peptide with sequence CQSRRCRKNAFQEL, from the internal region of the protein sequence according to NP_079496.1.
Specificity This antibody is expected to recognise isoform alpha (NP_079496.1) and not isoform beta (NP_694882.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ADAM33
Background Synonyms: ADAM33, a disintegrin and metalloproteinase domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, a disintegrin and metalloprotease 33, disintegrin and reprolysin metalloproteinase family protein, metalloprotease disintegrin, ADAM metallopeptidase domain 33, RP5-964F7.2, C20orf153, MGC149823
Gene ID 80332
NCBI Accession NP_079496
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Experiments gave bands at approx 80-90 kDa and strong ladder from 26 to 38 kDa in Human Prostate lysates after 0.3 µg/mL antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul,31(1):13-21, PMID: 14742294). This protein has a calculated MW of 87.7 kDa according to NP_079496.1. Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-ADAM Metallopeptidase Domain 33 (ADAM33) (Internal Region) antibody ABIN185401 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
General Blakey, Halapi, Bjornsdottir et al.: "Contribution of ADAM33 polymorphisms to the population risk of asthma." in: Thorax, Vol. 60, Issue 4, pp. 274-6, 2005 (PubMed).

Simpson, Maniatis, Jury et al.: "Polymorphisms in a disintegrin and metalloprotease 33 (ADAM33) predict impaired early-life lung function." in: American journal of respiratory and critical care medicine, Vol. 172, Issue 1, pp. 55-60, 2005 (PubMed).

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