Osteopontin (SPP1) antibody

Antigen: Osteopontin (SPP1)

Synonyms: OPN, BNSP, BSPI, ETA-1, MGC110940

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185425

Additional Information

Alternative name: Osteopontin / SPP1

Gene ID: 6696, 20750 (mouse)

Immunogen: C-DPKSKEEDKHLK

Format: Affinity-purified

Description: All names and symbols: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN , Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1. Official Symbol: SPP1. RefSeq number(s): NP_000573.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Hormones, Enzymes, Metabolism, Extracellular Matrix

Restrictions: For Research Use only

Images

ABIN185425 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kolb, Kleeff, Guweidhi et al.: "Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions." in: Cancer biology & therapy, Vol. 4, Issue 7, pp. 740-6, 2005 (PubMed).