Add to Basket
Order hotline:
 |
+1 404 474 4654 |
 |
+1 888 205 9894 (TF) |
Secreted phosphoprotein 1 (SPP1) antibody
| Antigen | Secreted phosphoprotein 1 (SPP1) |
| Synonyms | OPN, BNSP, BSPI, ETA-1, MGC110940, OP, Bsp, Eta, Opn, Ric, Opnl, Apl-1, Spp-1, AA960535, AI790405, OSP, SPP1, spp1, MGC111821 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185425 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
|
| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Osteopontin / SPP1 |
| Gene ID | 6696, 20750 (mouse) |
| Immunogen | C-DPKSKEEDKHLK |
| Format | Affinity-purified |
| Description | All names and symbols: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN , Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1. Official Symbol: SPP1. RefSeq number(s): NP_000573.1 |
| Specificity | Human, Mouse, Rat, Dog |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Hormones, Enzymes, Metabolism, Extracellular Matrix |
| Restrictions | For Research Use only |
Images
| ABIN185425 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Kolb, Kleeff, Guweidhi et al.: "Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions." in: Cancer biology & therapy, Vol. 4, Issue 7, pp. 740-6, 2005 (PubMed).
|
Alternatives
Alternatives for antigen "Secreted phosphoprotein 1 (SPP1)", type "Antibodies"
| Hosts | Rabbit (50), Mouse (45), Goat (6) |
| Reactivities | Human (74), Mouse (Murine) (29), Rat (Rattus) (25), Dog (Canine) (8), Pig (Porcine) (4) |
| Applications | Western Blotting (WB) (82), Enzyme Immunoassay (EIA) (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), ELISA (18), Flow Cytometry (FACS) (14), Immunofluorescence (IF) (13), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | C-Term (7), AA 164-179 (5), AA 59-74 (5), N-Term (5), AA 0-17 (3) |
