Complement Factor H (CFH) (Internal Region) antibody

Details for Product No. ABIN185437
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Antigen
Synonyms CFH, im:7147412, si:ch211-207o17.2, Mud-1, NOM, Sas-1, Sas1, AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, Fh, fH
Epitope
Internal Region
(12), (10), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(130), (20), (18), (2), (2), (1), (1)
Host
Goat
(65), (35), (18), (14), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(82), (64), (31), (20), (19), (15), (13), (11), (10), (10), (9), (8), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185437
374.27 $
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Immunogen Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.
Specificity This antibody is expected to recognize isoform a (NP_000177.2) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Complement factor H
Background Synonyms: complement factor H, HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H, complement factor H, isoform b, factor H, factor H-like 1
Gene ID 3075
NCBI Accession NP_000177
Research Area Complement Factors
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 170 kDa band observed in human lung lysates (calculated MW of 139 kDa according to NP_000177.2). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Complement Factor H (CFH) (Internal Region) antibody ABIN185437 staining (0.03µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Klein, Zeiss, Chew et al.: "Complement factor H polymorphism in age-related macular degeneration." in: Science (New York, N.Y.), Vol. 308, Issue 5720, pp. 385-9, 2005 (PubMed).

Validation Images
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