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OCT4 antibody (POU Class 5 Homeobox 1) (C-Term)

Details for Product anti-POU5F1 Antibody No. ABIN185438, Supplier: Log in to see
Antigen
  • POU5F1L
  • POU5F1
  • OCT3
  • OCT4
  • OTF-3
  • OTF3
  • OTF4
  • Oct-3
  • Oct-4
  • NF-A3
  • Oct-3/4
  • Oct3
  • Oct3/4
  • Oct4
  • Otf-3
  • Otf-4
  • Otf3
  • Otf3-rs7
  • Otf3g
  • Otf4
  • gp9
  • gp-9
  • oct4
  • pou2
  • POU-2
  • cb197
  • spg/pou2
  • chunp6868
  • wu:fd18d06
Epitope
C-Term
37
31
31
20
13
12
8
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
304
107
45
10
10
10
8
5
5
5
3
3
3
2
1
1
Host
Goat
215
92
29
11
Clonality
Polyclonal
Conjugate
This OCT4 antibody is un-conjugated
13
11
10
10
6
6
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
262
156
54
49
35
33
28
19
12
12
8
4
3
3
3
2
2
1
Supplier
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Immunogen Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2, NP_976034.3.
Blocking Peptide Blocking peptide for this product available: ABIN370032
Specificity This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name OCT4 / POU5F1 (POU5F1 Antibody Abstract)
Background Synonyms: POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, transcription factor 1
Gene ID 5460, 18999, 294562
NCBI Accession NP_976034, NP_002692
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38 kDa band observed in Human Liver lysates (calculated MW of 38.6 kDa according to NP_002692.2). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (C-Term) (ABIN185438) ABIN185438 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Pri...
Background publications Chew, Loh, Zhang et al.: "Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 6031-46, 2005 (PubMed).

Jones, Ulbright, Eble et al.: "OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma." in: The American journal of surgical pathology, Vol. 28, Issue 7, pp. 935-40, 2004 (PubMed).