V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) antibody

Antigen: V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyms: ERBB3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185461

Additional Information

Alternative name: ERBB3 / HER3

Gene ID: 2065, 13867 (mouse), 29496 (rat)

Immunogen: C-HSRLFPKANAQRT

Format: Affinity-purified

Description: All names and symbols: ERBB3, HER3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) , HGNC:3431, ErbB-3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 , erbB-3. Official Symbol: ERBB3. RefSeq number(s): NP_001973.2

Specificity: This antibody is expected to recognise isoform 1 (NP_001973.2) only. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150kDa band observed in human lysates of T cell line JURKAT (calculated MW of 148kDa according to NP_001973.2). Recommended concentration: 0.1-0.3µg/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185461 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: van der Horst, Weber, Ullrich: "Tyrosine phosphorylation of PYK2 mediates heregulin-induced glioma invasion: novel heregulin/HER3-stimulated signaling pathway in glioma." in: International journal of cancer. Journal international du cancer, Vol. 113, Issue 5, pp. 689-98, 2004 (PubMed).