PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Internal Region)

Details for Product anti-PDPK1 Antibody No. ABIN185505, Supplier: Log in to see
Antigen
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Alternatives
anti-Rat (Rattus) PDPK1 antibody for ELISA
Epitope
Internal Region
58
34
32
27
25
21
18
17
15
15
15
15
8
6
6
6
6
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
442
221
220
19
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Host
Goat
364
70
15
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
3
3
3
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
292
153
96
91
62
61
40
29
20
10
7
5
3
2
1
Options
Supplier
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Immunogen Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
Blocking Peptide Blocking peptide for this product available: ABIN368847
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Synonyms: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Gene ID 5163
NCBI Accession NP_002601
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 65-70 kDa band observed in Rat Heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Internal Region) (ABIN185505) ABIN185505 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Prima...
Background publications Sarbassov, Guertin, Ali, Sabatini: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).