anti-PDK1 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 1) (Internal Region)

Details for Product anti-PDK1 Antibody No. ABIN185505
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Antigen
Synonyms B830012B01, D530020C15Rik, si:zfos-979f1.3
Epitope
Internal Region
(34), (17), (13), (12), (10), (10), (6), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(206), (86), (85), (7), (4), (4), (3), (3), (1), (1), (1), (1)
Host
Goat
(167), (32), (15)
Clonality
Polyclonal
Conjugate
This PDK1 antibody is un-conjugated
(6), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(171), (97), (77), (26), (22), (12), (10), (10), (6), (4), (4)
Pubmed 1 reference available
Quantity 100 μg
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Immunogen Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PDK1 (PDK1 Antibody Abstract)
Background Synonyms: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Gene ID 5163
NCBI Accession NP_002601
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 65-70 kDa band observed in Rat Heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (Internal Region) antibody ABIN185505 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Prima...
Background publications Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

Validation Images
Catalog No. ABIN185505
325.97 $
Plus shipping costs $45.00
Quantity
Price
100 μg
325.97 $

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