Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (Internal Region) antibody

Details for Product No. ABIN185505
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Antigen
Synonyms B830012B01, D530020C15Rik, si:zfos-979f1.3
Epitope
Internal Region
(29), (11), (11), (11), (8), (7), (6), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(162), (87), (81), (25), (25), (24), (14), (12), (6), (1), (1), (1), (1)
Host
Goat
(137), (26), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(124), (73), (52), (20), (17), (13), (9), (6), (5), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN185505
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PDK1
Background Synonyms: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Gene ID 5163
NCBI Accession NP_002601
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 65-70 kDa band observed in Rat Heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (Internal Region) antibody ABIN185505 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

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