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PDK1 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 1) (Internal Region)

Details for Product anti-PDK1 Antibody No. ABIN185505, Supplier: Login to see
Antigen
  • B830012B01
  • D530020C15Rik
  • si:zfos-979f1.3
Alternatives
anti-Human PDK1 antibody for Western Blotting
Epitope
Internal Region
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16
16
10
7
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5
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
151
40
39
6
4
4
3
3
1
1
1
Host
Goat
120
22
14
Clonality
Polyclonal
Conjugate
This PDK1 antibody is un-conjugated
6
5
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3
3
3
2
2
2
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
127
77
41
13
9
5
5
5
3
2
1
1
Supplier
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Immunogen Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
Blocking Peptide Blocking peptide for this product available: ABIN368847
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PDK1 (PDK1 Antibody Abstract)
Background Synonyms: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Gene ID 5163
NCBI Accession NP_002601
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 65-70 kDa band observed in Rat Heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-PDK1 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 1) (Internal Region) (ABIN185505) ABIN185505 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Prima...
Background publications Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).