Eyes Absent Homolog 4 (Drosophila) (EYA4) antibody

Antigen: Eyes Absent Homolog 4 (Drosophila) (EYA4)

Synonyms: CMD1J, DFNA10, B130023L16Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185511

Additional Information

Alternative name: EYA4

Gene ID: 2070

Immunogen: EDSQDLNEQSVKKTC

Description: All names and symbols: EYA4, eyes absent homolog 4 (Drosophila) , HGNC:3522, DFNA10 , OTTHUMP00000040267, dJ78N10.1 (eyes absent (Drosophila) homolog 4), deafness, autosomal dominant 10, eyes absent 4. Official Symbol: EYA4. RefSeq number(s): NP_004091.3, NP_742102.2, NP_742103.1

Specificity: This antibody is expected to recognise isoforms a, c and d (NP_004091.2, NP_742102.1 and NP_742103.1 respectively). Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Schoenberger, Wang, Shin et al.: "Mutation in the transcriptional coactivator EYA4 causes dilated cardiomyopathy and sensorineural hearing loss." in: Nature genetics, Vol. 37, Issue 4, pp. 418-22, 2005 (PubMed).