Proline Dehydrogenase (Oxidase) 1 (PRODH) (Internal Region) antibody

Details for Product No. ABIN185594
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Antigen
Synonyms
MGC115247, GB16218, CG1417, Dmel\\CG1417, EE85, PRODH, sl, slg, tu3, ARABIDOPSIS THALIANA PROLINE OXIDASE, AT-POX, ATPDH, ATPOX, EARLY RESPONSIVE TO DEHYDRATION 5, PRO1, PROLINE DEHYDROGENASE, PROLINE ... show more
MGC115247, GB16218, CG1417, Dmel\\CG1417, EE85, PRODH, sl, slg, tu3, ARABIDOPSIS THALIANA PROLINE OXIDASE, AT-POX, ATPDH, ATPOX, EARLY RESPONSIVE TO DEHYDRATION 5, PRO1, PROLINE DEHYDROGENASE, PROLINE OXIDASE, Pro-1, Pro1, Ym24d07, HSPOX2, PIG6, POX, PRODH1, PRODH2, TP53I6 show less
Epitope
Internal Region
(12), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(36), (17), (12)
Host
Goat
(24), (9), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(21), (19), (10), (10), (3), (2)
Pubmed 1 reference available
Quantity 100 μg
Options
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Catalog No. ABIN185594
374.27 $
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Immunogen Peptide with sequence C-ERDGSGTNKRDKQYQ, from the internal region of the protein sequence according to NP_057419.2.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PRODH
Background Synonyms: PRODH, proline dehydrogenase (oxidase) 1, HSPOX2, PIG6, PRODH1, PRODH2, SCZD4, TP53I6, FLJ33744, MGC148078, MGC148079, Proline oxidase, mitochondrial, p53 induced protein, proline dehydrogenase (proline oxidase ), proline oxidase 2, tumor protein p53 inducible protein 6
Gene ID 5625, 19125
NCBI Accession NP_057419
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave bands at approx 85 kDa and 25 kDa in Human Brain lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 68.0 kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Paterlini, Zakharenko, Lai et al.: "Transcriptional and behavioral interaction between 22q11.2 orthologs modulates schizophrenia-related phenotypes in mice." in: Nature neuroscience, Vol. 8, Issue 11, pp. 1586-94, 2005 (PubMed).

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