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SMAD9 antibody (SMAD Family Member 9)

Details for Product anti-SMAD9 Antibody No. ABIN185609, Supplier: Log in to see
Antigen
  • SMAD8
  • MADH6
  • MADH9
  • PPH2
  • SMAD8A
  • SMAD8B
  • Madh8
  • Madh9
  • Smad8
Reactivity
Human
73
16
15
5
1
1
1
1
1
Host
Goat
46
18
6
4
Clonality
Polyclonal
Conjugate
This SMAD9 antibody is un-conjugated
3
3
3
3
3
3
1
Application
Enzyme Immunoassay (EIA), ELISA, Western Blotting (WB)
61
42
10
9
6
6
4
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.
Blocking Peptide Blocking peptide for this product available: ABIN369207
Specificity This antibody is not expected to cross-react with SMAD1 and SMAD5. Human, Mouse, Rat, Dog
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name SMAD9 (SMAD9 Antibody Abstract)
Background This antibody is not expected to cross-react with SMAD1 and SMAD5. Synonyms: SMAD9 antibody, SMAD, mothers against DPP homolog 9 (Drosophila)] antibody, HGNC:6774 antibody, MADH6 antibody, MADH9 antibody, SMAD8A antibody, SMAD8B antibody, MAD (mothers against decapentaplegic, Drosophila) homolog 9 antibody, MAD, mothers against decapentaplegic homolog 9 antibody, MAD, mothers against decapentaplegic homolog 9 (Drosophila) antibody, Mothers against decapentaplegic, drosophila, homolog of, 9 antibody.
Official Symbol: SMAD9.
Gene ID 4093
NCBI Accession NP_005896
Research Area Cancer, Signaling, Transcription Factors
Application Notes Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SMAD9 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-SMAD9 antibody (SMAD Family Member 9) (ABIN185609) ABIN185609 (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Pr...
Background publications Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).