GRP94 antibody (Internal Region)
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- Target See all GRP94 (HSP90B1) Antibodies
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GRP94 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
- Purpose
- GPR94 / TRA1
- Sequence
- KEGVKFDESE KTKE
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.
- Isotype
- IgG
- Top Product
- Discover our top product HSP90B1 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5 μg/mL.
Western Blot: Approx 100 kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5 kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecula
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
- Alternative Name
- HSP90B1 (HSP90B1 Products)
- Synonyms
- ecgp antibody, gp96 antibody, tra1 antibody, grp94 antibody, DDBDRAFT_0206450 antibody, DDBDRAFT_0215015 antibody, DDB_0206450 antibody, DDB_0215015 antibody, TRA1 antibody, HSP90B1 antibody, ERp99 antibody, GRP94 antibody, TA-3 antibody, Targ2 antibody, Tra-1 antibody, Tra1 antibody, endoplasmin antibody, GP96 antibody, fb61d09 antibody, tra-1 antibody, wu:fb61d09 antibody, wu:fq25g01 antibody, Grp94 antibody, ECGP antibody, Gp96 antibody, ppk98 antibody, hsp108 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, heat shock protein Hsp90 family protein antibody, 94 kD glucose-regulated protein antibody, heat shock protein 90 beta family member 1 antibody, heat shock protein 90, beta (Grp94), member 1 antibody, heat shock protein 90, beta (grp94), member 1 antibody, heat shock protein 90kDa beta family member 1 L homeolog antibody, hsp90b1 antibody, grp94 antibody, HSP90B1 antibody, Hsp90b1 antibody, hsp90b1.L antibody
- Background
- TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1
- Gene ID
- 7184, 22027, 362862
- NCBI Accession
- NP_003290
- Pathways
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
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