GRIN2B antibody (Internal Region)

Catalog No. ABIN185658

The Goat Polyclonal anti-GRIN2B antibody (ABIN185658) specifically detects GRIN2B in WB, ELISA and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for GRIN2B antibody (Internal Region) (ABIN185658)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-GRIN2B Antibody

Binding Specificity: Internal Region

Purpose: NMDAR2B / GRIN2B

Sequence: KDAHEKDDFH HLS

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDAHEKDDFHHLS, from the internal region of the protein sequence according to NP_000825.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 170 kDa band observed in Rat Brain lysates (calculated MW of 166 kDa according to NP_000825.1). Recommended concentration: 2-4 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoskeleton/ cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: GRIN2B

Background: GRIN2B, NMDAR2B, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, HGNC:4586, NR2B, hNR3 , N-methyl-D-aspartate receptor subunit 2B

Gene ID: 2904, 14812, 24410

NCBI Accession: NP_000825

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity