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Wilms Tumor 1 (WT1) (N-Term) antibody

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Antigen
Synonyms WT1, WT, XWT1a, wt-1, wt1, xWT1, gud, wagr, wt33, xwt1, wit-2, AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33, XWT1b, XeWT1, CWT1, WT-1, D630046I19Rik, Wt-1, si:ch211-160j6.3, zgc:152697
Epitope
N-Term
(22), (21), (14), (14), (11), (11), (9), (9), (9), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(136), (33), (30), (2), (1), (1), (1), (1), (1)
Host
Goat
(73), (48), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (4), (3), (3), (3), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(92), (48), (47), (41), (39), (26), (19), (5), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Availability Available
Immunogen Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence according to NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3.
Blocking Peptide Blocking peptide for this product available: ABIN370392
Specificity This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Wilms tumor 1 / WT1 (WT1 Antibody Abstract)
Background Synonyms: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33
Gene ID 7490
NCBI Accession NP_077743, NP_077742, NP_000369, NP_077744
Pathways
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 55 kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3 kDa according to NP_000369.3). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Wilms Tumor 1 (WT1) (N-Term) antibody (ABIN185674) ABIN185674 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). ...
Background publications Rao, Pham, Imam et al.: "Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis." in: Genes & development, Vol. 20, Issue 2, pp. 147-52, 2006 (PubMed).

Catalog No. ABIN185674
$318.20
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