Von Hippel-Lindau Tumor Suppressor (VHL) antibody

Antigen: Von Hippel-Lindau Tumor Suppressor (VHL)

Synonyms: RCA1, VHL1, HRCA1, Vhlh, BcDNA:RH61560, VHL, d-VHL, dVHL, dmVHL, Dmel\CG13221, CG13221, rca1, vhl1, hrca1, zgc:158722, DKFZp469K2019

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185675

Additional Information

Alternative name: VHL

Gene ID: 7428, 22346 (mouse), 24874 (rat)

Immunogen: C-RSLVKPENYRRLD

Format: Affinity-purified

Description: All names and symbols: VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome. Official Symbol: VHL. RefSeq number(s): NP_000542.1, NP_937799.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1). Human, Mouse, Rat, Dog 213 aa, 172 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 17kDa band observed in Human Ovary and Testis lysates and in lysates of all cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration:

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Hormones, Cytokines

Restrictions: For Research Use only

Images

ABIN185675 (0.01 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kong, Lin, Liang et al.: "Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha." in: Molecular and cellular biology, Vol. 26, Issue 6, pp. 2019-28, 2006 (PubMed).