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SEMA5A antibody (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A) (Internal Region)

Details for Product anti-SEMA5A Antibody No. ABIN185680, Supplier: Log in to see
Antigen
  • 5930434A13
  • 9130201M22Rik
  • AI464145
  • SEMAF
  • Semaf
  • semF
  • wu:fa95e03
  • wu:fi41a04
Epitope
Internal Region
19
16
15
11
2
2
2
2
1
1
Reactivity
Human
69
21
3
2
2
1
1
1
Host
Goat
57
14
3
3
Clonality
Polyclonal
Conjugate
This SEMA5A antibody is un-conjugated
7
7
7
7
7
7
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
64
60
12
3
1
1
Supplier
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Immunogen Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.
Blocking Peptide Blocking peptide for this product available: ABIN370073
Specificity The immunizing peptide corresponds to a part of the extracellular domain.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Semaphorin 5A (SEMA5A Antibody Abstract)
Background Synonyms: SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
Gene ID 9037, 20356, 310207
NCBI Accession NP_003957
Research Area Neurology
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 90 kDa in Mouse Brain lysates and approx 25 kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121 kDa according to NP_003957.1. The 90 kDa and 25 kDa band were successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Maraganore, de Andrade, Lesnick, Strain, Farrer, Rocca, Pant, Frazer, Cox, Ballinger: "High-resolution whole-genome association study of Parkinson disease." in: American journal of human genetics, Vol. 77, Issue 5, pp. 685-93, 2005

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