SEMA5A antibody (Internal Region)
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- Target See all SEMA5A Antibodies
- SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SEMA5A antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC)
- Purpose
- Semaphorin 5A
- Sequence
- DEATKKACYS KGKS
- Specificity
- The immunizing peptide corresponds to a part of the extracellular domain.
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.
- Isotype
- IgG
- Top Product
- Discover our top product SEMA5A Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6 μg/mL.
Western Blot: Preliminary experiments gave an approx 90 kDa in Mouse Brain lysates and approx 25 kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanati
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
- Alternative Name
- SEMA5A (SEMA5A Products)
- Synonyms
- wu:fa95e03 antibody, wu:fi41a04 antibody, SEMAF antibody, semF antibody, 5930434A13 antibody, 9130201M22Rik antibody, AI464145 antibody, Semaf antibody, semaphorin 5A antibody, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A antibody, semaphorin-5A antibody, SEMA5A antibody, sema5a antibody, Sema5a antibody, LOC100516117 antibody
- Background
- SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
- Gene ID
- 9037, 20356, 310207
- NCBI Accession
- NP_003957
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