Carnitine Palmitoyltransferase 1A (Liver) (CPT1A) antibody

Antigen: Carnitine Palmitoyltransferase 1A (Liver) (CPT1A)

Synonyms: CPT1, CPT1-L, L-CPT1, CPTI, Cpt1, C730027G07, MGC53498

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185710

Additional Information

Alternative name: CPT1A

Gene ID: 1374

Immunogen: C-DPAQTVEQRLKLFK

Format: Affinity-purified

Description: All names and symbols: CPT1A, carnitine palmitoyltransferase 1A (liver) , CPT1, CPT1-L, L-CPT1 , carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase I, carnitine palmitoyltransferase I, liver. Official Symbol: CPT1A. RefSeq number(s): NP_001867.2, NP_001027017.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_001867.2, NP_001027017.1) but is not expected to recognise CPT1B. Human 773 aa, 756 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 80kDa band observed in human liver lysates (calculated MW of 86.2kDa according to NP_001027017.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Fatty Acids, Metabolism, Organelles

Restrictions: For Research Use only

Images

ABIN185710 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Pocai, Lam, Obici et al.: "Restoration of hypothalamic lipid sensing normalizes energy and glucose homeostasis in overfed rats." in: The Journal of clinical investigation, Vol. 116, Issue 4, pp. 1081-91, 2006 (PubMed).