CPT1A antibody (Middle Region)

Catalog No. ABIN2782010

There are 3+ publications for this product available. The Rabbit Polyclonal anti-CPT1A antibody is suitable to detect CPT1A in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for CPT1A antibody (Middle Region) (ABIN2782010)

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPT1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CPT1A Antibody

Binding Specificity: Middle Region

Purpose: CPT1A Antibody - middle region

Sequence: LSTSQTPQQQ VELFDLENNP EYVSSGGGFG PVADDGYGVS YILVGENLIN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CPT1A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CPT1A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44796-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CPT1A antibody (ABIN2782010)

Piemontese, Cerchia, Laghezza, Ziccardi, Sblano, Tortorella, Iacobazzi, Infantino, Convertini, Dal Piaz, Lupo, Colantuoni, Lavecchia, Loiodice: "New diphenylmethane derivatives as peroxisome proliferator-activated receptor alpha/gamma dual agonists endowed with anti-proliferative effects and mitochondrial activity." in: European journal of medicinal chemistry, Vol. 127, pp. 379-397, (2017) (PubMed).

Convertini, Menga, Andria, Scala, Santarsiero, Castiglione Morelli, Iacobazzi, Infantino: "The contribution of the citrate pathway to oxidative stress in Down syndrome." in: Immunology, Vol. 149, Issue 4, pp. 423-431, (2016) (PubMed).

Laghezza, Pochetti, Lavecchia, Fracchiolla, Faliti, Piemontese, Di Giovanni, Iacobazzi, Infantino, Montanari, Capelli, Tortorella, Loiodice: "New 2-(aryloxy)-3-phenylpropanoic acids as peroxisome proliferator-activated receptor ?/? dual agonists able to upregulate mitochondrial carnitine shuttle system gene expression." in: Journal of medicinal chemistry, Vol. 56, Issue 1, pp. 60-72, (2013) (PubMed).

Target Details for CPT1A

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Alternative Name: CPT1A

Background:

Background Information: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: Carnitine palmitoyltransferase 1A (liver)

Alternative Symbols: CPT1, CPT1-L, L-CPT1

Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform

Molecular Weight: 88 kDa

Gene ID: 1374

NCBI Accession: NP_001027017

UniProt: P50416

Pathways: AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process