CD166 antibody (AA 28-527)
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- Target See all CD166 (ALCAM) Antibodies
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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Binding Specificity
- AA 28-527
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD166 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM)
- Sequence
- MGHHHHHHSG SEF-WYT VNSAYGDTII IPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL NLSENYTLSI SNARISDEKR FVCMLVTEDN VFEAPTIVKV FKQPSKPEIV SKALFLETEQ LKKLGDCISE DSYPDGNITW YRNGKVLHPL EGAVVIIFKK EMDPVTQLYT MTSTLEYKTT KADIQMPFTC SVTYYGPSGQ KTIHSEQAVF DIYYPTEQVT IQVLPPKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL TDVRRNATGD YKCSLIDKKS MIASTAITVH YLDLSLNPSG EVTRQIGDAL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADEISDENRE KVNDQAK
- Specificity
- The antibody is a mouse monoclonal antibody raised against ALCAM. It has been selected for its ability to recognize ALCAM in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM) corresdonding to Trp28~Lys527 (Accession # Q13740)
- Clone
- 5-2#
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ALCAM Primary Antibody
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- Application Notes
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Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
- Alternative Name
- Activated Leukocyte Cell Adhesion Molecule (ALCAM Products)
- Synonyms
- CD166 antibody, MEMD antibody, ALCAM antibody, BEN antibody, DM-GRASP antibody, JC7 antibody, SC1 antibody, AI853494 antibody, MuSC antibody, alcam antibody, cd166 antibody, memd antibody, fb53h09 antibody, fb80a10 antibody, nlcam antibody, si:ch211-190h2.4 antibody, wu:fb53h09 antibody, wu:fb80a10 antibody, wu:fc14b01 antibody, activated leukocyte cell adhesion molecule antibody, activated leukocyte cell adhesion molecule a antibody, activated leukocyte cell adhesion molecule L homeolog antibody, CD166 antigen homolog antibody, activated leukocyte cell adhesion molecule b antibody, ALCAM antibody, Alcam antibody, alcama antibody, alcam.L antibody, alcam antibody, cd166 antibody, alcamb antibody
- Background
- CD166, MEMD, Activated Leucocyte Cell Adhesion Molecule
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