CA12 (AA 161-318) antibody

Details for Product No. ABIN1858202, Supplier: Log in to see
Antigen
  • CAXII
  • HsT18816
  • CAR12
  • 2310047E01Rik
  • AI314958
  • Ca12
  • carbonic anhydrase XII
  • carbonic anyhydrase 12
  • CA12
  • Car12
Epitope
AA 161-318
12
11
10
7
5
5
5
4
4
4
3
2
2
1
1
1
Reactivity
Mouse (Murine)
117
35
29
1
1
Host
Rabbit
76
42
13
Clonality
Polyclonal
Conjugate
Un-conjugated
13
12
7
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
79
63
19
15
13
8
7
7
5
5
3
2
1
1
Options
Supplier
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Available images

'Independent Validation' Badge
Antigen CA12
Lot Number A20170109527
Method validated Immunohistochemistry
Positive Control mouse kidney, mouse ciliary body
Negative Control secondary antibody only
Primary Antibody ABIN3204699
Secondary Antibody goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
Protocol
  • Cryo-section:
    • Wash mouse eye and kidney tissue in PBS.
    • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
    • Gently freeze suspended tissue in liquid nitrogen for 5min.
    • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
    • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
    • Rinse sections in PBS 3 times for 5min.
    • Block sections with 5% normal goat serum for 1h at RT.
  • Immunostaining:
    • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
    • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
    • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
    • Counterstain with DAPI in the mounting medium step.
  • Wash and mount:
    • Rinse sections in PBS 3 times for 5min.
    • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
  • Microscopy:
    • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
Validation Images
Immunohistochemistry validation image for anti-CA12 (AA 161-318) antibody (ABIN3204699) Immunostaining of murine kidney tissue (A.) and ciliary body sections (B.) with the C...
Immunogen CA12 (Ala161-Ile318)
Isotype IgG
Specificity The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Alternative Name Carbonic Anhydrase XII (CA12)
Background Alternative Names: CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
Application Notes
  • Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only
Format Liquid
Concentration 200 μg/mL
Buffer 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-CA12 (AA 161-318) antibody (ABIN1858202) anti-CA12 (AA 161-318) antibody