CA12 antibody (AA 188-199)

Catalog No. ABIN1781864

Product Details anti-CA12 Antibody

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Binding Specificity: AA 188-199

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: carbonic anhydrase XII (aa188-199)

Sequence: SHLQHVKYKG QE

Specificity: This antibody is expected to recognize all reported isoforms (NP_001209.1, NP_996808.1, NP_001280571.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of 39.5 kDa according to NP_001209.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CA12

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: CA12 (CA12 Products)

Synonyms: CAXII antibody, HsT18816 antibody, CAR12 antibody, 2310047E01Rik antibody, AI314958 antibody, Ca12 antibody, ca12 antibody, carbonic anhydrase 12 antibody, carbonic anhydrase XII L homeolog antibody, CA12 antibody, Car12 antibody, ca12.L antibody

Background: CA12, carbonic anhydrase XII, CAXII, HsT18816, CA-XII, carbonate dehydratase XII, carbonic anhydrase 12, carbonic dehydratase, tumor antigen HOM-RCC-3.1.3

Molecular Weight: Expected molecular weight: 45 kDa

Gene ID: 771

NCBI Accession: NP_001209, NP_996808, NP_001280571