MIB2 antibody (AA 687-930)

Catalog No. ABIN1859821

Product Details anti-MIB2 Antibody

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Binding Specificity: AA 687-930

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Mindbomb Homolog 2 (MIB2)

Specificity: The antibody is a rabbit polyclonal antibody raised against MIB2. It has been selected for its ability to recognize MIB2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mindbomb Homolog 2 (MIB2) corresdonding to Thr687~Leu930 (Accession # Q96AX9)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MIB2

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Alternative Name: Mindbomb Homolog 2 (MIB2 Products)

Background: ZZANK1, SKD, ZZZ5, Skeletrophin, Novelzin, Novel zinc finger protein, Zinc Finger,ZZ Type With Ankyrin Repeat Domain 1, Putative NF-kappa-B-activating protein, Mind bomb 2

Pathways: Notch Signaling