MIB2 antibody

Catalog No. ABIN7229664

The Rabbit Polyclonal anti-MIB2 antibody is suitable to detect MIB2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MIB2 antibody (ABIN7229664)

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MIB2 Antibody

Purpose: Rabbit Anti-MIB2 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MIB2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MIB2 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MIB2

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Alternative Name: MIB2

Background: E3 ubiquitin-protein ligase MIB2, Mind bomb homolog 2, Novel zinc finger protein, Novelzin, Putative NF-kappa-B-activating protein 002N, RING-type E3 ubiquitin transferase MIB2, Skeletrophin, Zinc finger ZZ type with ankyrin repeat domain protein 1Mindbomb E3 Ubiquitin Protein Ligase 2 encoded by MIB2 is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion.

Molecular Weight: 111kD

Gene ID: 142678

UniProt: Q96AX9

Pathways: Notch Signaling