KIT Ligand antibody (AA 24-247) (FITC)
-
- Target See all KIT Ligand (KITLG) Antibodies
- KIT Ligand (KITLG)
-
Binding Specificity
- AA 24-247
-
Reactivity
- Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This KIT Ligand antibody is conjugated to FITC
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Sequence
- MGHHHHHHSG S-KTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRA
- Specificity
- It has been selected for its ability to recognize SCF in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
-
Recombinant SCF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SCF conjugated to fitc. - Isotype
- IgG
- Top Product
- Discover our top product KITLG Primary Antibody
-
-
- Application Notes
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
-
Content: The quality control contains recombinant SCF (Lys24~Ala247) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
-
- Target
- KIT Ligand (KITLG)
- Alternative Name
- Stem Cell Factor (KITLG Products)
- Synonyms
- Xkl-1 antibody, Xsl antibody, Xsl-1 antibody, Xsl-2 antibody, steel antibody, KITLG antibody, SCF antibody, kitl antibody, kl-1 antibody, mgf antibody, scf antibody, FPH2 antibody, KL-1 antibody, Kitl antibody, MGF antibody, SF antibody, SHEP7 antibody, Clo antibody, Con antibody, Gb antibody, Kitlg antibody, Mgf antibody, SLF antibody, Sl antibody, blz antibody, contrasted antibody, 710-712 antibody, CSF antibody, KITL antibody, KIT ligand L homeolog antibody, KIT ligand antibody, kit ligand antibody, kitlg.L antibody, KITLG antibody, kitlg antibody, Kitl antibody, Kitlg antibody
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
-