FAS antibody

Catalog No. ABIN187302

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody is specific for human Fas. Species Reactivity: Human. Other species not tested.

Immunogen: Mouse L cells stably over-expressing human Fas.

Clone: 2R2

Isotype: IgG3

Application Details

Application Notes: Functional activity: Induces apoptosis, no cross-linking required. Can be used in DISC (death-inducing signaling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. Flow cytometry The optimal dilution for a specific application should be determined by the researcher. Figure 1. Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells. Method: (A) Dose response: Jurkat cells (1x105) were treated with agonistic anti-Fas 2R2 antibody at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry. (B) Time course of apoptosis: Jurkat cells (1x105) were treated with 1 µg/mL anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA. Figure 2. Detection of Fas surface expression by FACS analysis. Method: Normal L cells (open histogram) and cells stably over-expressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunostaining was obtained with an isotype matched IgG control.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µg monoclonal antibody at 1 mg/mL purified from concentrated hybridoma tissue culture supernatant. Prepared in PBS containing protein stabilizer. Protein A-affinity purified. Purity 95% by SDS-PAGE.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity