Anti-MRP-14 clone 47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Using Western blot analysis 47-8D3 was found to detect a single protein band of 14 kDa in lysates of human monocytes and granulocytes, which was demonstrated to be the calcium-binding protein MRP14. Species Reactivity: Human. Others not tested.
Immunogen
A BALB/c mouse was immunized with human peripheral blood monocyte components. Splenocytes were fused with mouse myeloma cell line NS-1.
Western blot Immunohistochemistry (Frozen and paraffin) Staining strongly enhanced after digestion with Trypsin). ELISA This antibody may be used for phenotypical characterization of macrophages and myelomonocytic cells in situ. The optimal dilution for a specific application should be determined by the researcher.