HSP70 antibody

Catalog No. ABIN187606

Product Details anti-HSP70 Antibody

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This clone recognizes both the constitutive (Hsp73) and inducible (Hsp72) forms of Hsp70. Species Reactivity: Reacts with Human, Monkey and Bovine. Other species not tested. Positive Control: BT474 or HeLa cells. Breast carcinoma.

Immunogen: Hsp70 protein from HeLa cells.Produced by fusion between BALB/c spleno-cytes and mouse myeloma NS-1 cells.

Clone: W27

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry Immunofluorescence (Not assessed) Immunoprecipitation: Use at 2 µg/mg protein lysate. Use Protein A. Western blotting: 0.5-1 µg/ml for 2 hrs. at RT Immunohistology: Formalin/paraffin - Use at 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Clone W27 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100ug/500ul in 10 mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Purified from ascites fluid by Protein A chromatography.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Heat shock protein 70 / HSP70 (HSP70 Products)

Synonyms: APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody

Background: A wide variety of environmental and patho-physiological stress conditions trigger the synthesis of a family of proteins known as heat shock proteins, more appropriately called stress response proteins. Many heat shock proteins, including members of the Ssp70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation and secretion. Hsp70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other unknown proteins. Also, Hsp70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs and other damaging conditions.