S1PR5 antibody (C-Term)

Catalog No. ABIN187790

The Rabbit Polyclonal anti-S1PR5 antibody is suitable to detect S1PR5 in samples from Human. It has been validated for WB.

Quick Overview for S1PR5 antibody (C-Term) (ABIN187790)

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-S1PR5 Antibody

Binding Specificity: C-Term

Specificity: Reacts with EDG-8. Species Reactivity: Human, other species not tested.

Sterility: Sterile filtered

Immunogen: Synthetic peptide derived from the C-terminus of the human EDG-8 protein.

Application Details

Application Notes: Western Blot: Use at 5-10 µg/mL using a human EDG-8 receptor transfected cell line. Detects recombinant EDG-8 receptors with a band of about 42 kDa. [ Note: Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for Western blot development.] The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Purified sterile filtered polyclonal antibody against human EDG-8 (CT). 100 µg/100 µL in PBS with 0.08% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for S1PR5

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Alternative Name: EDG-8 / S1P5

Background: Endothelial Cell Differentiation Gene-8 (EDG-8) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-8 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-8 is expressed in oligodendrocytes and fibrous astrocytes in the rat brain.