S1PR5 antibody (Extracellular, N-Term)

Catalog No. ABIN7887145

The Rabbit Polyclonal anti-S1PR5 antibody is suitable to detect S1PR5 in samples from Mouse. It has been validated for WB, FACS and LCI.

Quick Overview for S1PR5 antibody (Extracellular, N-Term) (ABIN7887145)

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-S1PR5 Antibody

Binding Specificity: AA 24-39, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 5

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-S1PR5/EDG8 (extracellular) Antibody (ABIN7043777, ABIN7045276 and ABIN7045277) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize EDG8 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KLRGARYQPGAGLRAD, corresponding to amino acid residues 24-39 of mouse S1PR5 (EDG8)

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237280)

Blocking Peptide: (ABIN7237280)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for S1PR5

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Alternative Name: S1PR5

Background:

Synonyms: S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Lysophospholipid receptor B4, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8

Description: Sphingosine-1-phosphate receptor 5 also known as S1PR5 (EDG8) is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P).Lysophospholipids encompass a broad range of small, membrane-derived phospholipids that act as extracellular signals, mediated through specific receptors1. S1PR5 is a high-affinity cell surface S1P receptor, a member of the S1PR family. Like all GPCRs it has the classical structure of seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal1.The signaling pathways triggered by S1P/S1P binding play a critical regulatory role in many pathophysiological processes, including skeletal muscle and nervous system degeneration, inflammation, cancer, and autoimmune disorders and hence this interaction has important implications for clinical applications and for personalized medicine2,3. Targeting S1P receptors and their downstream signaling cascades is considered as a potential treatment for cancer and various autoimmune diseases4.

Gene ID: 94226

NCBI Accession: NM_030760

UniProt: Q91X56