S1PR5 antibody (N-Term)

Catalog No. ABIN2775524

This Rabbit Polyclonal antibody specifically detects S1PR5 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig and Cow.It has been mentioned in 1 publication

Quick Overview for S1PR5 antibody (N-Term) (ABIN2775524)

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-S1PR5 Antibody

Binding Specificity: N-Term

Purpose: EDG8 Antibody - N-terminal region

Sequence: MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA VVCLAVCAFI

Predicted Reactivity: Cow: 80%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EDG8. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EDG8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112686

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-S1PR5 antibody (ABIN2775524)

Bock, Pfalzgraff, Weindl: "Sphingosine 1-phospate differentially modulates maturation and function of human Langerhans-like cells." in: Journal of dermatological science, Vol. 82, Issue 1, pp. 9-17, (2016) (PubMed).

Target Details for S1PR5

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Alternative Name: EDG8

Background:

Background Information: EDG8 is a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. It Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). It may play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Gene Name: Sphingosine-1-phosphate receptor 5

Alternative Symbols: EDG8, S1P5, Edg-8, SPPR-1, SPPR-2

Protein Name: Sphingosine 1-phosphate receptor 5

Molecular Weight: 16kDa

Gene ID: 53637

UniProt: Q9H228