CHEK2 antibody (N-Term)

Catalog No. ABIN1881196

This Rabbit Polyclonal antibody specifically detects CHEK2 in WB, IF, IHC (p) and FACS. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for CHEK2 antibody (N-Term) (ABIN1881196)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB19067

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 111-141, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-CHEK2 antibody (ABIN1881196)

Xiao, Zheng, Huang, Liu, Zhang, Ma: "Deficiency of 53BP1 inhibits the radiosensitivity of colorectal cancer." in: International journal of oncology, Vol. 49, Issue 4, pp. 1600-8, (2017) (PubMed).

Yao, Huang, Zheng, Liu, Lin, Zhang, Yang, Zhang, Ma: "53BP1 loss induces chemoresistance of colorectal cancer cells to 5-fluorouracil by inhibiting the ATM-CHK2-P53 pathway." in: Journal of cancer research and clinical oncology, Vol. 143, Issue 3, pp. 419-431, (2017) (PubMed).

Yang, Wood, Hrushesky: "Mammalian TIMELESS is required for ATM-dependent CHK2 activation and G2/M checkpoint control." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3030-4, (2010) (PubMed).

Varmark, Kwak, Theurkauf: "A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 312-20, (2010) (PubMed).

Zhu, Miao, Huang, Feng, Zhang, Lu, Cai, Tong, Xu, Qian, Ding: "Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 11, pp. 1226-34, (2009) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2

Background: CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53.

Molecular Weight: 60915

NCBI Accession: NP_001005735, NP_001244316, NP_009125, NP_665861

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle