This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This COL10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human COL10A1.
COL10A1
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Mäkitie, Susic, Cole: "Early-onset metaphyseal chondrodysplasia type Schmid associated with a COL10A1 frame-shift mutation and impaired trimerization of wild-type α1(X) protein chains." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 28, Issue 11, pp. 1497-501, (2010) (PubMed).
Wang, Qiu, Ma, Xia, Zhu, Zhu: "Expression of Runx2 and type X collagen in vertebral growth plate of patients with adolescent idiopathic scoliosis." in: Connective tissue research, Vol. 51, Issue 3, pp. 188-96, (2010) (PubMed).
Videman, Saarela, Kaprio, Näkki, Levälahti, Gill, Peltonen, Battié: "Associations of 25 structural, degradative, and inflammatory candidate genes with lumbar disc desiccation, bulging, and height narrowing." in: Arthritis and rheumatism, Vol. 60, Issue 2, pp. 470-81, (2009) (PubMed).
Higashikawa, Saito, Ikeda, Kamekura, Kawamura, Kan, Oshima, Ohba, Ogata, Takeshita, Nakamura, Chung, Kawaguchi: "Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type X collagen gene." in: Arthritis and rheumatism, Vol. 60, Issue 1, pp. 166-78, (2009) (PubMed).
Zimmermann, Boeuf, Dickhut, Boehmer, Olek, Richter: "Correlation of COL10A1 induction during chondrogenesis of mesenchymal stem cells with demethylation of two CpG sites in the COL10A1 promoter." in: Arthritis and rheumatism, Vol. 58, Issue 9, pp. 2743-53, (2008) (PubMed).
Col10 antibody, Col10a-1 antibody, collagen, type X, alpha 1 antibody, collagen type X alpha 1 chain antibody, Col10a1 antibody, COL10A1 antibody
Background
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).