Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
Pr, M, Pig, Rb, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
PPP2R2A
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
IF: 1:25. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Li, Cai, Shouse, Piluso, Liu: "A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55." in: The EMBO journal, Vol. 26, Issue 2, pp. 402-11, (2007) (PubMed).
Nusbaum, Mikkelsen, Zody, Asakawa, Taudien, Garber, Kodira, Schueler, Shimizu, Whittaker, Chang, Cuomo, Dewar, FitzGerald, Yang, Allen, Anderson, Asakawa, Blechschmidt, Bloom, Borowsky, Butler, Cook et al.: "DNA sequence and analysis of human chromosome 8. ..." in: Nature, Vol. 439, Issue 7074, pp. 331-5, (2006) (PubMed).
Mayer, Hendrix, Cron, Matthies, Stone, Goris, Merlevede, Hofsteenge, Hemmings: "Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform." in: Biochemistry, Vol. 30, Issue 15, pp. 3589-97, (1991) (PubMed).
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.