Endonuclease G antibody (N-Term)

Catalog No. ABIN1888159

Product Details anti-Endonuclease G Antibody

Target: Endonuclease G (ENDOG)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Affinity chromatography purified via peptide column

Immunogen: EndoG antibody was raised with a Synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: EndoG (ENDOG Products)

Synonyms: CG8862 antibody, Dmel\\CG8862 antibody, Tb08.10J17.140 antibody, Tb08.10J17.90 antibody, TBC1D13 antibody, endog antibody, ENDOG antibody, wu:fb79c08 antibody, zgc:110020 antibody, Endonuclease G antibody, endonuclease G antibody, putative endonuclease G antibody, endonuclease G, mitochondrial antibody, endonuclease G L homeolog antibody, EndoG antibody, Tc00.1047053506867.10 antibody, Tb927.8.4040 antibody, Tb927.8.4090 antibody, LBRM_10_0750 antibody, LMJF_10_0610 antibody, NAEGRDRAFT_78507 antibody, ENDOG antibody, endog antibody, eNDOG antibody, LOC100529218 antibody, Endog antibody, endog.L antibody

Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death.The activities of caspase and nuclease are involved in the DNA fragmentation.Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45.Caspase and CAD independent DNA fragmentation also exists.Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD.EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C.elegans.EndooG also cleaves DNA in vitro.

Molecular Weight: 35 kDa

NCBI Accession: NP_004426

Pathways: Apoptosis