APH1A antibody (Internal Region)

Catalog No. ABIN190762

This Goat Polyclonal antibody specifically detects APH1A in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for APH1A antibody (Internal Region) (ABIN190762)

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APH1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-APH1A Antibody

Binding Specificity: Internal Region

Purpose: APH1A

Sequence: HVTDRSDARL QYG

Specificity: This antibody is expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HVTDRSDARLQYG, from the internal region of the protein sequence according to NP_001071096.1, NP_057106.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 26.8 kDa according to NP_057106.2). Recommended concentration: 0.05-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APH1A

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Alternative Name: APH1A

Background: APH1A, anterior pharynx defective 1 homolog A (C. elegans), 6530402N02Rik, APH-1A, CGI-78 , anterior pharynx defective 1 homolog A

Gene ID: 51107

NCBI Accession: NP_001071096, NP_057106

Pathways: Notch Signaling, Neurotrophin Signaling Pathway