NR0B1 antibody (Internal Region)
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- Target See all NR0B1 Antibodies
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NR0B1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- DAX1 / NR0B1
- Sequence
- CGEDHPQQGS TLY
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence CGEDHPQQGSTLY, from the internal region of the protein sequence according to NP_000466.2.
- Isotype
- IgG
- Top Product
- Discover our top product NR0B1 Primary Antibody
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- Application Notes
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Western Blot: Approx 55 kDa band observed in Human Adrenal Gland lysates (calculated MW of 51.7 kDa according to NP_000466.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
- Alternative Name
- NR0B1 (NR0B1 Products)
- Synonyms
- DAX antibody, NR0B1 antibody, dax1 antibody, AHC antibody, AHCH antibody, AHX antibody, DAX-1 antibody, DAX1 antibody, DSS antibody, GTD antibody, HHG antibody, NROB1 antibody, SRXY2 antibody, Ahc antibody, Ahch antibody, Dax1 antibody, nuclear receptor subfamily 0 group B member 1 antibody, nuclear receptor subfamily 0, group B, member 1 antibody, nuclear receptor subfamily 0 group B member 1 L homeolog antibody, NR0B1 antibody, nr0b1 antibody, nr0b1.L antibody, Nr0b1 antibody
- Background
- NR0B1, DAX1, nuclear receptor subfamily 0, group B, member 1, AHC, AHCH, AHX, DAX-1, DSS, GTD, HHG, NROB1 , dosage-sensitive sex reversal, gonadotropin deficiency, nuclear hormone receptor
- Gene ID
- 190
- NCBI Accession
- NP_000466
- Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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