immunophilin homolog ARA9 (AIP) antibody

Antigen: immunophilin homolog ARA9 (AIP)

Synonyms: ARA9, XAP2, FKBP16, FKBP37, SMTPHN

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190790

Additional Information

Alternative name: AIP / ARA9

Gene ID: 9049, 11632 (mouse), 282827 (rat)

Immunogen: C-EARIRQKDEEDKAR

Format: Affinity-purified

Description: All names and symbols: AIP, ARA9, aryl hydrocarbon receptor interacting protein , FKBP16, FKBP37, XAP2 , HBV-X associated protein, aryl hydrocarbon receptor-interacting protein. Official Symbol: AIP. RefSeq number(s): NP_003968.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.02-0.06µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190790 (0.02µg/ml) staining of NIH/3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ARA9 (RC211157) and probed with ABIN190790 (mock transfection in first lane), tested by Origene.

Publications

Publications: Vierimaa, Georgitsi, Lehtonen et al.: "Pituitary adenoma predisposition caused by germline mutations in the AIP gene." in: Science (New York, N.Y.), Vol. 312, Issue 5777, pp. 1228-30, 2006 (PubMed).