AIP antibody (AA 91-330)

Catalog No. ABIN6719423

The Mouse Monoclonal anti-AIP antibody is suitable to detect AIP in samples from Human, Rat, Mouse and Monkey. It has been validated for WB, IHC, FACS, ICC and IF.

Quick Overview for AIP antibody (AA 91-330) (ABIN6719423)

Target: AIP (Aryl Hydrocarbon Receptor Interacting Protein (AIP))

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This AIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 10G8

Product Details anti-AIP Antibody

Binding Specificity: AA 91-330

Purpose: Anti-ARA9/AIP Antibody (monoclonal, 10G8)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse,rat ARA9.

Characteristics: Anti-ARA9/AIP Antibody.(monoclonal, 10G8). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ARA9 recombinant protein (Position: D91-H330). Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AIP

Target: AIP (Aryl Hydrocarbon Receptor Interacting Protein (AIP))

Alternative Name: AIP

Background:

Background: AIP, also known as, ARA9 or XAP-2, is a protein that in humans is encoded by the AIP gene. This gene is mapped to 11q13.2. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. AIP may play a positive role in aryl hydrocarbon receptor-mediated signalling possibly by influencing its receptivity for ligand and/or its nuclear targeting. It has been shown that AIP is the cellular negative regulator of the hepatitis B virus (HBV) X protein. AIP mutations may be the cause of a familial form of acromegaly, familial isolated pituitary adenoma (FIPA).

Gene Full Name: AHR interacting HSP90 co-chaperone

Molecular Weight: 38 kDa

Gene ID: 9049

UniProt: O00170