Interferon Induced with Helicase C Domain 1 (IFIH1) antibody

Antigen: Interferon Induced with Helicase C Domain 1 (IFIH1)

Synonyms: Hlcd, MDA5, MDA-5, IDDM19, MGC133047

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA

Catalog no.: ABIN190791

Additional Information

Alternative name: MDA5 / IFIH1

Gene ID: 71586 (mouse)

Immunogen: C-KQKLRNNDLKKQN

Description: All names and symbols: Ifih1, interferon induced with helicase C domain 1, 9130009C22Rik, Helicard, Hlcd, MDA-5, MDA5, MGC90959, melanoma differentiation associated protein-5. Official Symbol: Ifih1. RefSeq number(s): NP_082111.1

Specificity: Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_082111.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).